6.1产品详情

Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.

6.1.1 精密度：

Intra-assay Precision (Precision within an assay): CV%<15%  

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%  

Three samples of known concentration were tested in twenty assays to assess.

6.1.2 标准曲线

6.1.3 本试剂盒所含材料

· A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.

· Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.

· Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.

· HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.

· HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.

· Sample Diluent (2 x 20 ml) --Dilute the sample solution.

· Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.

· TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.

· Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.

· Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.

· An Instruction manual

本试剂盒不含材料：

· A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

· An incubator that can provide stable incubation conditions up to 37°C±5°C.

· Centrifuge

· Vortex

· Squirt bottle, manifold dispenser, or automated microplate washer.

· Absorbent paper for blotting the microtiter plate.

· 50-300ul multi-channel micropipette

· 100ml and 500ml graduated cylinders.

· Deionized or distilled water.

· Pipette tips

· Timer

· Test tubes for dilution.

6.1.4储存方法：-20℃保存

6.2 COA界面

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6.3 MSDS界面

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